Part:BBa_K737012:Design

gvpC-GFP fusion protein UH with medium constitutive promoter

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 1265
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal SpeI site found at 1265
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 1265
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 1265
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1187
  

Design Notes

We referred to the paper:New Fusion Protein Systems Designed to Give Soluble Expression in Escherichia coli Gregory D. Davis,* Claude Elisee, Denton M. Newham, Roger G. Harri Received 26 January 1999; accepted 16 May 1999

Source

Yes.